中文版　　 实验室主页
Dr. Xin-Hui Xing
Professor and Director
Institute of Biochemical Engineering
Email:xhxing@ tsinghua.edu.cn

 

 

[Education]

B.S. South China University of Technology (1985),

M.S. Utsunomiya University, Japan (1989),

PhD. Tokyo Institute of Technology, Japan (1992)

 

[Work Experiences]

April, 1992 to February, 1998 

Assistant Professor, Department of Bioscience and Biotechnology,

Tokyo Institute of Technology of Japan

March, 1998 to July, 2001

Lecturer and Associate Professor, Department of Material Science and Chemical Engineering, Yokohama National University of Japan

September, 2001 to Now

     Professor selected by 100-talent Scholars Programme, Department of Chemical Engineering, Tsinghua University, Beijing

September, 2002 to now

     Director of Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University

January, 2004 to now

     Vice director of Research Institute of Pharmaceuticals, Institute of Medical Science

 

[Current Research Interests]

His research field covers bioenergy, environmental biotechnology, enzyme engineering and nanobiotechnology. Before joining Tsinghua University in 2001, he had ever worked in universities of Japan for ten years. His current research interests include: 1) development of Rapid and visible quantification tools for bioprocess control and optimization by using fusion protein technology; 2) real-time functional analysis and construction of a multi-species microbial coupling systems 3) construction of novel NADH regeneration systems and their applications; 4) functional analysis of new hydrogenases and their applications; 5) cell membrane engineering and control of electron transport across the cells; 6) genetic engineering of methanotrophs and the reconstructions of metabolic pathway; 7) production of soluble heparinase I fused with affinity tags and the enzymatic process for clean production of low molecular weight heparin; 8) system biological engineering of Streptomyces sps.; 9) metabolic and bioprocess engineering for enhanced biohydrogen production with higher H2 yield from biomass; 10) mechanism of atmospheric-pressure low temperature plasma acting on microorganisms and the plasma biotechnology; 11) high cell density culture of methanotrophes and their bioindustry applications; 12) biological systems for wastewater treatment capable of on-site reduction of excess sludge; 13) application of microbubble system in industrial and environmental biotechnology.

 

[Teaching Courses]

1.       Freshman Seminar (Undergraduates)

2.       Environmental Biotechnology (Graduates)

3.       International advanced Biological Engineering (Graduates)

 

[Social Activities]

1.       Membership of Division of Biochemical Engineering, Chinese Society of Chemical Engineering

2.       Membership of Division of Enzyme Engineering, Chinese Society of Microbiology

3.       Associate editor of Biochemical Engineering Journal

4.       Editorial board of Journal of Molecular Catalysis B: Enzymatic

5.       Editorial board of Journal of Biological Engineering

6.       Editorial board of Enzymatic and Microbial Technology

7.       Editorial board of Biotechnology and Business of China

8.       Editorial board of Journal of Food Sciences of China

9.       Editorial board of Journal of Food Science and Biotechnology

 

[Selected representative publications in recent 5 years]

(English papers)

1) Chong Zhang, Xin-Hui Xing and Min-Sheng Liu, Production of Multienzymes Consisting of Alkaline Amylase and Cellulase by Mixed Alkalophilic Culture and their Potential Use in the Saccharification of Sweet Potato. Biochemical Engineering Journal, 19, 181-187 (2004)

2）S. Yamamoto, M. Katsui, X.-H. Xing and H. Unno, Relations between cell spreading state and G-CSF productivity of recombinant CHO. Biochemical Engineering Journal, 20, 57-60 (2004)

3) Chen Yin, Xin-Hui Xing and Lou Kai, Construction of Recombinant E. coli for Over-Production of Soluble Heparinase I by Fusion to Maltose-Binding Protein. Biochemical Engineering Journal, 23, 155-159 (2005)

4) Yunli Ren, Xing-Hui Xing, Chong Zhang and ZhongXuan Gou, A simplified method for assay of hydrogenase activities of H2 evolution and uptake in Enterobacter aerogenes. Biotechnology Letters, 27 (14), 1029-1033 (2005)

5) Chong Zhang, Xin-Hui Xing and Kai Lou, Rapid Detection of a gfp-marked Enterobacter aerogenes under anaerobic conditions by aerobic fluorescence recovery (AFR). FEMS Microbiology Letters, 249, 211-218 (2005)

6) Xing Xin-Hui, Wu Hao, Luo Ming-Fang and Wang Bang-Peng, effects of Organic Chemicals  Growth of Methylosinus trichosporium OB3b. Biochemical Engineering Journal, 31, 113-117（2006）

7）Zhongxuan Gou, Xin-Hui Xing, Mingfang Luo, Hao Jiang, Bing Han, Hao Wu, Lei Wang and Fei Zhang, Functional Expression of Particulate Methane Monooxygenase Gene in Recombinant Rhodococcus erythropolis. FEMS Microbiology Letters, 263,136-141 (2006)

8) Mingshu Xu, Mingfang Luo, Xin-Hui Xing and Hongzhang Chen, Characteristics of quercetin transglycosidation catalyzed by Penicillium decumbens glycosidase. Food and Bioproducts Processing, 84(C3),237-241 (2006)

9) Ying Kuang, Xin-Hui Xing, Yin Chen, Fengchun Ye, Yu Chen, Yiyang Yang, Zheng Liu and Ruchang Bi, Production of Heparin Oligosaccharides by Fusion Protein of MBP-Heparinase I and the Enzymatic Thermostability. Journal of Molecular Catalysis B: Enzymatic. 43, 90-95 (2006)

10) Anfeng Yu, Quan Feng, Zehua Liu, Yunan Zhou and Xin-Hui Xing, Biological wastewater treatment by a bioreactor with repeatedly coupling of aerobes and anaerobes aiming at on-site reduction of excess sludge. Water Science and Technology, 53(9), 71-77 (2006)

11) Yin Chen, Xin-Hui Xing, Fengchun Ye and Ying Kuang, Rapid quantification of heparinase I activity by green fluorescent protein in recombinant Escherichia coli. Chinese J. Chem. Eng., 15(1), 122-126 (2007)

12) Feng-yi Su, Yumie Endo, Hiroshi Saiki, Xin-Hui Xing and Naoya Ohmura, Simple and Sensitive Bacterial Quantification by a Flow-based Kinetic Exclusion Fluorescence Immunoassay. Biosensors and Bioelectronics, 22, 2500-2507 (2007)

13) Yin Chen and Xin-Hui Xing, Fengchun Ye, Ying Kuang, and Mingfang Luo, Production of MBP-HepA fusion protein in recombinant Escherichia coli by optimization of culture medium. Biochemical Engineering Journal, 34, 114-121 (2007)

14) Li-Bing Chu, Xin-Hui Xing, An-Feng Yu, Yu-Nan Zhou, Xu-Lin Xun and Benjamin Jurcik, Enhanced ozonation of dyestuff wastewater by microbubbles. Chemosphere, 68, 1854-1860 (2007)

15) Quan Feng, Anfeng Yu, Libing Chu and Xin-Hui Xing, Performance study of the reduction of excess sludge and simultaneous removal of organic carbon and nitrogen by a combination of fluidized- and fixed-bed bioreactors with different structured macroporous carriers. Biochemical Engineering Journal, 39, 344-352(2008)

16）Guobin Shan, Wenbiao Jin, Edward Lam, Xin-Hui Xing, Purification of total DNA extracted from activated sludge. Journal of Environmental Science, 20, 1-7 (2008)

17）Yunan Zhou, Xin-Hui Xing, Zehua Liu, Liwen Cui, Anfeng Yu, Quan Feng and Haijun Yang, Enhanced coagulation with iron salts aided by tannic acid for phosphorus removal from wastewater and biologically treated wastewater. Chemosphere, 72(2), 290-298 (2008)

28) Li-Bing Chu, Sang-Tian Yan, Xin-Hui Xing, An-Feng Yu, Xu-Lin Sun, Benjamin Jurcik, Enhanced Sludge Solubilization by Microbubble Ozonation. Chemosphere, 72, 205-212 (2008)

19) Chong Zhang and Xin-Hui Xing, Quantification of a specific bacterial strain in an anaerobic mixed culture for biohydrogen production by the aerobic fluorescence recovery (AFR) technique. Biochemical Engineering Journal, In press (2008)

20) Guo Li, He-Ping Li, Li-Yan Wang, Sen Wang, Hong-Xin Zhao, Wen-Ting Sun, Xin-Hui Xing, Cheng-Yu Bao, Genetic effects of radio-frequency, atmospheric-pressure glow discharges with helium. APPLIED PHYSICS LETTERS, 92, 221504 (2008)

21) Sangtian Yan, Kazuhiko Miyanaga, Xin-Hui Xing and Yasunori Tanji, Succession of bacterial community and enzymatic activities of activated sludge by heat-treatment for reduction of excess sludge. Biochemical Engineering Journal, 39, 598-603 (2008)

22) Li-Bing Chu, Xin-Hui Xing, An-Feng Yu, Xu-Lin Sun, Benjamin Jurcik, Enhanced Treatment of Practical Textile Wastewater by Microbubble Ozonation. Process Safety and Environmental Protection, in press (2008)

 

（Chinese Papers）

23)刘元帅，沈金玉，邢新会，余立新，李春，曹竹安， 电渗析技术在发酵液脱盐中的应用研究， 高校化学工程学报，18(2)，174-178（2004）

24)韩 云，邢新会，杨敏，刘敏胜，娄恺，绿色荧光蛋白在酵母有机废水处理功能解析中应用的初探研究。环境科学，25(1),40-（2003）

25)苏凤宜，邢新会，孙旭临，过氧乙酸溶解剩余污泥的研究。高校化学工程学报，18（4），471-476（2004）

26)娄 恺， 于安峰， 邢新会，重组大肠杆菌生产极端耐热木聚糖酶。过程工程学报，4（4）：501-506（2004）

27)邢新会，刘则华，环境生物修复技术的研究进展。化工进展，23（6），579-584 （2004）

28）张翀, 邢新会，辅酶再生体系的研究进展。生物工程学报（Chinese Journal of Biotechnology），20（6），811-816（2004）

29）     冯权，邢新会，刘则华，以剩余污泥减量化为目标的废水生物处理技术研究进展。化工进展，23（8），832-836（2004）

30）     张翀,邢新会, 发酵生物制氢研究进展。生物加工过程，3,1-8（2005）

31）     赵雪冰 苏凤宜 邢新会，过氧乙酸氧化木质素磺酸钠的研究。清华大学学报，1244-1247（2005）

32）     许明淑,罗明芳,邢新会,黄璐琦,酶法强化中药提取的研究进展。中国中医药信息杂志，12（12），37-39（2005）

33）     刘则华，邢新会，冯权，污水中磷的化学去除及回收利用。上海工程技术大学学报，19(2),125-128(2005)

34）     刘敏胜，邢新会，专性嗜碱芽孢杆菌Bacillus pseudofirmus的基因导入方法。化工学报，57,922-926（2006）

35）     刘则华，邢新会，冯权，利用多孔微生物载体的固定床生物反应器的污水处理特性。水处理技术，32(4),34-38（2006）

36）     刘敏胜，韩冰，邢新会，荧光蛋白在枯草芽孢杆菌中的表达方法研究。清华大学学报，接收（2006）

37）     冯权、刘则华、梁鹏，邢新会，用好氧－厌氧反复耦合生物反应器处理肌苷生产废水。化工环保，26(6)，484-487（2006）

38）     叶逢春，陈银，邢新会，重组大肠杆菌生产可溶性MBP融合肝素酶的培养条件优化。生物加工过程，4（3），28-32（2006）

39）     许明淑，邢新会，罗明芳，黄璐琦，银杏叶黄酮的酶法强化提取工艺条件研究。中国实验方剂学杂志，12（4），2-4（2006）

40）     单国彬，金文标，林佶侃，邢新会，变性梯度凝胶电泳在环境微生物生态学中的应用。生态学杂志，25（10），1257-1264（2006）

41）     罗明芳，吴昊，王磊，邢新会，含有甲烷氧化菌的混合菌群特性研究。微生物学报，47（1）：108-114（2007）

42）     陈银、叶逢春、况莹、邢新会，肝素酶研究进展。中国生物工程杂志，已接受（2007）

43）     初里冰，邢新会，于安峰，孙旭林，本杰明，微米气泡强化臭氧氧化的作用机理研究。环境化学，26（5），622-625（2007）

44）     郝利民, 邢新会, 张延坤, 等. 裂褶菌发酵液化学成分分析及其抗缺氧效果研究, 食品科学, 2007, 28（7）：321～324.

45）     张建峰，苏凤宜，邢新会，邻苯二酚2,3-双加氧酶在恶臭假单胞杆菌整细胞催化中的酶活检测方法，化工学报，59(2), 450-455(2008)

46）     王海胜，阮志勇，卢元，邢新会，姜瑞波，紫色杆菌素研究进展。化工进展，27（3），315-321（2008）

47）     王海胜，卢元，薛园，阮志勇，姜瑞波，邢新会，娄恺，杜檊氏菌B2的蓝色素成分的分离及化学结构解析。化工学报，59（3），630－635（2008）

48）     赵洪新，来奇恒，杨程，张子能，邢新会，产气肠杆菌磷酸酶基因克隆表达及其特性。化工学报，59（8），2071－2078（2008）(EI收录)

49）     韩冰，苏涛，李信，邢新会，甲烷氧化菌及甲烷单加氧酶的研究进展。生物工程学报（Chinese Journal of Biotechnology），印刷中（2008）

 

（Patents）

34 Patents have been filed and among which more than 10 have been approved.

 

（Industrial Applications）

1．Biological Wastewater Treatment Technology Capable of Reducing Excess Sludge by Repeatedly Cooperation of Aerobes and Anaerobes (rCAA) have been applied in treatment of domestic sewage, river pollutions, decentralized wastewater in rural regions and industrial wastewater, and proved to be effective and economical.

2．Production of MBP-fused heparinase I and its application for enzymatic clean production of low molecular weight heparin (LMWH) has been succeeded and is coming into the stage of pilot study in industry. This technology has great potential for pharmaceutical industries.